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Perimed Inc periscan pim 3 system
Periscan Pim 3 System, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/periscan+pim+3/3+doppler+imager+laser+perfusion+pim/pmc12785507-85-19-26
Average 86 stars, based on 1 article reviews
periscan pim 3 system - by Bioz Stars, 2026-09
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Article Title: Evaluation of Bromelain-Based Enzymatic Debridement Combined with Laser Doppler Imaging and Healing of Burn Wounds
Article Snippet: LDI scans were done using PeriScan PIM 3 (Perimed AB, Stockholm, Sweden).

Article Title: Reproducibility of high-resolution laser speckle contrast imaging to assess cutaneous microcirculation for wound healing monitoring in mice.
Article Snippet: Objective: Laser speckle contrast imaging (LSCI) combines an excellent spatial and temporal resolution, with excellent reproducibility in humans.. Recently, high-resolution LSCI (LSCI-HR), coupled or not with oximetry, have been marketed.. They are promising approaches to assess wound healing, especially in rodents.

Article Title: A comprehensive classification and analysis of oily sensitive facial skin: a cross-sectional study of young Chinese women
Article Snippet: , Blood Flow Perfusion , PeriScan PIM 3 (Perimed AB, Stockholm, Sweden) , Cheek.

Article Title: Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose‐Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration
Article Snippet: Hindlimb blood perfusion was assessed on days 0, 3, 7, and 14 after surgery using PeriScan PIM 3 (Perimed AB, Stockholm, Sweden).

In Vivo:

Article Title: Deferoxamine topical cream superior to patch in rescuing radiation‐induced fibrosis of unwounded and wounded skin
Article Snippet: .. A PeriScan PIM 3 (Perimed, Järfälla, Sweden) laser Doppler was used to scan for perfusion within the dorsal skin in vivo. ..

Article Title: Deferoxamine topical cream superior to patch in rescuing radiation-induced fibrosis of unwounded and wounded skin.
Article Snippet: .. A PeriScan PIM 3 (Perimed, Järfälla, Sweden) laser Doppler was used to scan for perfusion within the dorsal skin in vivo. ..

Imaging:

Article Title: A comprehensive classification and analysis of oily sensitive facial skin: a cross-sectional study of young Chinese women.
Article Snippet: .. Scientific Reports | (2025) 15:1633 3| https://doi.org/10.1038/s41598-024-85000-z (A) Descriptive Statistics Parameter category Parameter name Testing Equipment and Specifications Testing Area Directly Collected by Instrument Skin Moisture Content (CM) Corneometer CM 825 (Courage + Khazaka, Germany) Cheek Transepidermal Water Loss (TEWL) Tewameter TM300 (Courage + Khazaka, Germany) Cheek Sebum Content (SM) Sebumeter SM815 (Courage + Khazaka, Germany) forehead, canthus, cheek, and jaw Melanin (MEXA)/Eryth (ERYTH)MEXA/ERYTH Mexameter MX18 (Courage + Khazaka, Germany) Cheek Skin Colour (ITA°)/Luminosity (L*)/Redness (a*)/Yellowness (b*)ITA°/b* Colorimeter CL400 (Courage + Khazaka, Germany) Cheek Glossiness (GLOSS_DSC) Skin-Glossymeter GL200 Cheek Skin Elasticity (R2/R5/R7) Cutometer MPA580 (Courage + Khazaka, Germany) Cheek Skin Roughness (Ra/Rp/Rz) VISIO 3D (DERMATOP V3, France) Cheek Current Perception Threshold (CPT2000 Hz/CPT250 Hz/CPT50 Hz) NEUROMETER CPT/C (Neurotron, Germany) Cheek Blood Flow Perfusion PeriScan PIM 3 (Perimed AB, Stockholm, Sweden) Cheek Skin pH Skin pH-meter PH905 (Courage + Khazaka, Germany) Cheek Extracted by Imaging Red Area/Spots: Surface average, Perimeter average, Occupation rate, Irregularity of edge, Spot AVG blue/green/red, SpotL, spotA, spotB, Spot AVG grayscale, Skin AVG blue/red/green/L/A/B, Skin AVG grayscale, Homogeneity Haralick Extracted after obtaining standard facial images with Visia-CR Entire face Brightness (Oily): Lightness, Specular Brightness Rate, Diffused Brightness Rate, Global Brightness Rate Wrinkles (Forehead/Glabellar/Crow’s Feet/Eye Fine Lines/Nasolabial Folds/Oral Furrows): Coefficient of visibility, Occupancy rate, Contrast wrinkles/skin Follicular orifices: Number, Area average, Area variance, Follicular orifice area/AOI area, Diameter average, Diameter variance Porphyrin Value (on the nose in the middle of the face) Acne: Number of different parts/different forms (B) Parameter Meanings and Descriptive Statistics of Oily Sensitive Skin Assessment Parameter Set. ..

Article Title: Thymosin beta-4 improves endothelial function and reparative potency of diabetic endothelial cells differentiated from patient induced pluripotent stem cells
Article Snippet: .. A PeriScan PIM 3 (Perimed, Sweden), a laser Doppler imaging system, was used to visualize limb perfusion as described [ , ]. ..



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Perimed Inc periscan pim 3 system
Periscan Pim 3 System, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/periscan+pim+3/3+doppler+imager+laser+perfusion+pim/pmc12785507-85-19-26
Average 86 stars, based on 1 article reviews
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Laser Doppler Perfusion Imaging Periscan Pim 3, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Periscan Pim 3, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Perimed Inc laser doppler perfusion imaging system periscan pim 3
MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Laser Doppler Perfusion Imaging System Periscan Pim 3, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Lsci Periscan Pim 3 System, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative <t>LDPI</t> images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.
Laser Doppler Blood Flow Analyzer Periscan Pim 3 System, supplied by Perimed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/periscan+pim+3/laser+doppler+flowmetry+periflux+system+5000/us12208120-58-8-18
Average 90 stars, based on 1 article reviews
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Image Search Results


MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative LDPI images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.

Journal: Bioactive Materials

Article Title: Mechanosignaling and 3D morphological adaptation of MSCs in response to hydrogel rigidity underpin angiogenic and immunomodulatory efficacy for ischemic injury regeneration

doi: 10.1016/j.bioactmat.2025.07.027

Figure Lengend Snippet: MSCs modulated in SC hydrogels promote functional recovery in a murine hindlimb ischemia model. (a) Representative LDPI images of murine hindlimb ischemia model mice transplanted with PBS, hydrogel-only (Hydrogel), MSCs cultured on tissue culture plastic dishes (MSC), soft SC hydrogel encapsulating MSCs (Hydrogel + MSCs), and soft SC hydrogel encapsulating MSCs pretreated with PP2 (Hydrogel + MSCs + PP2) at 0, 3, 7, 14, 21, and 28 days post-operation. (b) Quantification of blood perfusion ratio from LDPI images. Data are presented as the mean ± SEM (n = 5). ∗∗ p < 0.01. (c) Representative photographs of ischemic limbs at day 28 post-operation. (d) Immunofluorescence staining of PCNA (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (e) Quantification of the number of PCNA-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (f) Immunofluorescence staining of cleaved caspase-3 (red) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (g) Quantification of the number of cleaved caspase-3-positive cells. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (h) Immunofluorescence staining of F4/80 (green) in ischemic limbs at day 3 post-operation. Scale bar = 50 μm. (i) Quantification of the number of F4/80-positive cells. Data are presented as the mean ± SEM (n = 3). ∗∗ p < 0.01 vs. PBS, and # p < 0.05 and ## p < 0.01 vs. Hydrogel + MSCs. (j) Immunofluorescence staining of CD31 (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (k) Quantification of the number of CD31-positive cells (capillary density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs. (l) Immunofluorescence staining of α-SMA (green) in ischemic limbs at day 28 post-operation. Scale bar = 50 μm. (m) Quantification of the number of α-SMA-positive cells (arteriole density) in ischemic tissues. Data are presented as the mean ± SEM (n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 vs. PBS, and ## p < 0.01 vs. Hydrogel + MSCs.

Article Snippet: Hindlimb blood flow was accessed using and LDPI PeriScan PIM 3 (Perimed, Järfälla, Sweden) by analyzing the ratio of blood flow in the ischemic limb (left) to the non-ligated limb (right) limb at 0, 3, 7, 14, 21, and 28 days of post-operation using PIMSoft (Perimed).

Techniques: Functional Assay, Cell Culture, Immunofluorescence, Staining